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KMID : 0368419870300040287
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Journal of Plant Biology
1987 Volume.30 No. 4 p.287 ~ p.298
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Culture and Fusion of Protoplasts from Potato (Solanum tuberosum L.) and Tobacco (Nicotiana tabacum L.)
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Chung, Sang Ho/鄭相浩
Sim, Woong Seop/沈雄燮
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Abstract
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The seasonal pattern and the annual magnitude of the internal (or biochemical) and plant-soil cycles of phosphorus in adjacent three different herb plant populations-Helianthus tuberosus L., Artemisia princeps Pampan and Phalaris arundinacea L. -in an old field of the basin in the Mt. Geumoh were investigated. A lot of the phosphorus demand for the three populations was supplied by the withdrawal from below-ground organ in early growing period, by the absorption from soil in mid-growing period and by the withdrawal from stem in later growing period. But in the A. princeps and P. arundinacea populations, a great deal of phosphorus seemed to be absorbed prior to the first withdrawal. The annual amount of the phosphorus flowing through the internal cycle was about 59% of the total phosphorus flow on the organ level in the H. tuberosus population, 41% in the A. princeps population and 32% in the P. arundinacea population, indicating that the tuber plant had the most developed internal cycle system. The annual amount of phosphorus which took part in the plant-soil cycle in the stand of the three populations was in the range of 4.49-5.65 gP m^-2, corresponding to only 3-8% of the extractable phosphorus contained in the soil of 0-20 ㎝ depth The fact that the H. tuberosus population is higher in the extent of internal cycle but smaller in the magnitude of plant-soil cycle and lower in the turnover rate of phosphorus than the other two populations suggests that the growth of H. tuberosus population may be less dependent on soil phosphorus availability than those of the other two populations.
The regenerative capacities of protoplasts isolated from potato(Solanum tuberosum L.) tubers and tobacco (Nicotiana tabacum L.) mesophyll tissues were examined, and then their intergeneric protoplast fusion was carried out. The potato tuber-derived protoplasts proliferated into the calli some of which showed rudimentary shoot-like structures, which had not been attempted before from tubers, while the tobacco protoplasts were regenerated into the whole plants. Intergeneric protoplast fusion between potato and tobacco was carried out and the heteroplasmic fusion products were formed. The first cell division of some of them was observed after 5 days of culture.
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